The Yeast Plasma Membrane Abc Transporter Aus1: Purification, Characterization and the Effect of Lipids on Its Activity

نویسندگان

  • Magdalena Marek
  • Sigrid Milles
  • Gabriele Schreiber
  • David L. Daleke
  • Gunnar Dittmar
  • Andreas Herrmann
  • Peter Müller
  • Thomas Günther Pomorski
چکیده

Institute of Biology, Humboldt University of Berlin, Invalidenstraße 42, 10115 Berlin, Germany; Department of Biochemistry and Molecular Biology, Indiana University, Bloomington, Indiana 47405, USA; Max-Delbrück Centrum for Molecular Medicine, Robert-Rössle Straße 10, 13125 Berlin, Germany; Center for Membrane Pumps in Cells and Disease PUMPKIN, Danish National Research Foundation, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Denmark; Present address: EMBL Heidelberg, Meyerhofstraße 1, 69117 Heidelberg, Germany

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Purification and characterisation of the yeast plasma membrane ATP binding cassette transporter Pdr11p

The ATP binding cassette (ABC) transporters Pdr11p and its paralog Aus1p are expressed under anaerobic growth conditions at the plasma membrane of the yeast Saccharomyces cerevisiae and are required for sterol uptake. However, the precise mechanism by which these ABC transporters facilitate sterol movement is unknown. In this study, an overexpression and purification procedure was developed wit...

متن کامل

An alarming biochemical side effect of Teucrium polium on the plasma membrane alkaline phosphatase of k562 cell line

The unexpected enhancement of alkalinee phosphatase (ALP) activity in the serum of a group of STZ-diabetic rats recieving the Teucrium polium extract (with hypoglycaemic property) may be attributed to its probable side effects. In order to get a more precise knowledge about this subject, we evaluated the effect of T. polium extract on the plasma membrane ALP activity of K562 cell line. Our data...

متن کامل

An alarming biochemical side effect of Teucrium polium on the plasma membrane alkaline phosphatase of k562 cell line

The unexpected enhancement of alkalinee phosphatase (ALP) activity in the serum of a group of STZ-diabetic rats recieving the Teucrium polium extract (with hypoglycaemic property) may be attributed to its probable side effects. In order to get a more precise knowledge about this subject, we evaluated the effect of T. polium extract on the plasma membrane ALP activity of K562 cell line. Our data...

متن کامل

Purification and Partial Characterization of a Thrombin-Like Enzyme (AH144) from Venom of Iranian Snake Agkistrodon Halys

The snake venom´s thrombin-like enzymes comprise a number of serine proteases, which are functionally and structurally related to thrombin. Purification and partial characterization of a thrombin-like enzyme from the venom of the Iranian snake, Agkistrodon halys, was the aim of this study. Purification was carried out by a combination of variety of chromatographic methods that included: gel...

متن کامل

Early induction of phenolic compounds in aluminum-treated roots of Brassica oleracea L. Roya Saghian1 and Faezeh Ghanati2*

There are evidences showing that adverse impacts of aluminum (Al) on plants growth and development are associated with its effect on plasma membrane and cell wall. Influence of Al on peroxidation of membrane lipids, wall-bound phenolics, lignin, soluble phenolics and the activity of certain enzymes involved in metabolism of phenols, i.e., phenylalanine ammonia lyase (PAL) and polyphenol oxidase...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2011